Control:Article Title: BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse BIRC2 Novus Biologicals Cat# NB100-56889 Anti-mouse b-Actin Santa Cruz Biotechnology Cat# sc-47778 Anti-mouse CD3 BioLegend Cat# 102102 Anti-mouse CD3 Novus Biologicals Cat# FAB4841G-100 Anti-mouse CD4 Novus Biologicals Cat# FAB554A-100 Anti-mouse CD8A Novus Biologicals Cat# NBP1-49045PE Anti-mouse CD11b Novus Biologicals Cat# NB110-89474AF405 Anti-mouse CD11c Novus Biologicals Cat# NB110-40766AF488 Anti-mouse CD25 Novus Biologicals Cat# NBP2-27425AF488 Anti-mouse CD28 BioLegend Cat# 100223 Anti-mouse CD44 Novus Biologicals Cat# NBP1-47386APC Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF488 Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF405 Anti-mouse CD69 Novus Biologicals Cat# NBP1-28011AF488 Anti-mouse CD80 Novus Biologicals Cat# NBP1-43385AF488 Anti-mouse CD314 Novus Biologicals Cat# FAB1547V-100UG Anti-mouse F4/80 Novus Biologicals Cat# NB600-404APC Anti-mouse FoxP3 Novus Biologicals Cat# NB100-39002PE Anti-human HIF-1a Novus Biologicals Cat# NB100-479 Anti-human HIF-1b Novus Biologicals Cat# NB100-124 Anti-human HIF-2a Novus Biologicals Cat# NB100-122 Anti-mouse IFNG Novus Biologicals Cat# IC485V-100UG Anti-mouse Ly6c Novus Biologicals Cat# NBP1-28046AF488 Anti-mouse Ly6g Novus Biologicals Cat# FAB1037A-100 Anti-mouse NK1.1 Novus Biologicals Cat# NB100-77528APC Anti-mouse p50 Novus Biologicals Cat# NBP2-6735 Anti-mouse Rel A Novus Biologicals Cat# NB100-2176 Anti-mouse Rel B Novus Biologicals Cat# NBP2-20123 Anti-mouse a-Tubulin Novus Biologicals Cat# NB600-506 Armenian Hamster IgG, anti-mouse CXCL9 (MIG) Bio X Cell Cat# BE0309 Polyclonal Armenian hamster IgG Bio X Cell Cat# BE0091 Syrian Hamster IgG, anti-mouse CTLA-4 Bio X Cell Cat# BP0131 Rat IgG2a, k, anti-mouse PD-1 (CD279) Bio X Cell Cat# BP0146 Rat IgG2a isotype control Bio X Cell Cat# BE0089 Chemicals, Peptides, and Recombinant Proteins Acriflavine Sigma Aldrich SKU # A8126 TRIzol Reagent Invitrogen Cat# 15596026 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 ECL Prime Western Blotting System GE Healthcare SKU# GERPN2232 PolyJet In Vitro DNA Transfection Reagent Signagen Cat # SL100688 Rabbit anti-mouse IgG-HRP Santa Cruz Biotech Cat# sc-358914 Rabbit IgG HRP Linked Whole Ab GE Healthcare SKU# GENA934 (Continued on next page) e1 Cell Reports 32, 108073, August 25, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase type I Sigma Aldrich Cat# SCR103 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) BD PharMingen Cat# 553141 Critical Commercial Assays High-Capacity RNA-to-cDNA Kit Applied Biosystem Cat# 4387406 Ambion DNase I (RNase-free) Invitrogen Cat# AM2222 iQ SYBR Green Supermix Bio-Rad Cat# 1708884 Mouse CXCL9/MIG Quantikine ELISA Kit R&D Cat# MCX900 RT2 ProfilerTM PCR Array of Mouse Cytokines & Chemokines QIAGEN Cat# PAMM-150ZD-6 Experimental Models: Cell lines MDA-MB-231 human breast cancer cell line ATCC ATCC HTB-26 4T1 mouse mammary carcinoma cell line ATCC ATCC CRL-2539 EMT-6 mouse mammary carcinoma cell line ATCC ATCC CRL-2755 B16F10 mouse melanoma cell line ATCC ATCC CRL-6475 293T human embryonic kidney cell line ATCC ATCC CRL-11268 Experimental Models: Organisms/Strains BALB/c female mice, 6-8 weeks old Charles River 028 C57BL/6 female mice, 6-8 weeks old Charles River 027 BALB/c Scid female mice, 6-8 weeks old The Jackson Laboratory 001803 Oligonucleotides m BIRC2 TGTGGCCTGATGTTGGATAAC ThermoFisher N/A m BIRC2 GGTGACGAATGTGCAAATCTACT ThermoFisher N/A m GPI TCAAGCTGCGCGAACTTTTTG ThermoFisher N/A m GPI GGTTCTTGGAGTAGTCCACCAG ThermoFisher N/A m ITGAV CCGTGGACTTCTTCGAGCC ThermoFisher N/A m ITGAV CTGTTGAATCAAACTCAATGGGC ThermoFisher N/A m MCL1 GACGACCTATACCGCCAGTC ThermoFisher N/A m MCL1 AGAGGCTTCGAGTCCTTGGA ThermoFisher N/A m NAMPT GCAGAAGCCGAGTTCAACATC ThermoFisher N/A m NAMPT TTTTCACGGCATTCAAAGTAGGA ThermoFisher N/A m SOD2 CAGACCTGCCTTACGACTATGG ThermoFisher N/A m SOD2 CTCGGTGGCGTTGAGATTGTT ThermoFisher N/A m UGP2 AGCAAAGCTATGTCTCAAGATGG ThermoFisher N/A m UGP2 GAGGCTGCTGTGGTAAGTATTT ThermoFisher N/A m RPL13A GGGCAGGTTCTGGTATTGGAT ThermoFisher N/A m RPL13A GGCTCGGAAATGGTAGGGG ThermoFisher N/A m TIGIT GAATGGAACCTGAGGAGTCTCT ThermoFisher N/A m TIGIT AGCAATGAAGCTCTCTAGGCT ThermoFisher N/A m LAG3 CTGGGACTGCTTTGGGAAG ThermoFisher N/A m LAG3 GGTTGATGTTGCCAGATAACCC ThermoFisher N/A m CD27 ATGCTTCGAGGATGGGGTG ThermoFisher N/A m CD276 CCAGGCTCTGGGGAAAAGG ThermoFisher N/A m 18S rRNA GTAACCCGTTGAACCCCATT ThermoFisher N/A m 18S rRNA CCATCCAATCGGTAGTAGCG ThermoFisher N/A h BIRC2 AGCACGATCTTGTCAGATTGG ThermoFisher N/A h BIRC2 GGCGGGGAAAGTTGAATATGTA ThermoFisher N/A h 18S rRNA CGGCGACGACCCATTCGAAC ThermoFisher N/A h 18S rRNA GAATCGAACCCTGATTCCCCGTC ThermoFisher N/A ChIP BIRC2 TCACACCTGTAATCCCAGCA ThermoFisher N/A (Continued on next page) Cell Reports 32, 108073, August 25, 2020 e2
Recombinant:Article Title: BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse BIRC2 Novus Biologicals Cat# NB100-56889 Anti-mouse b-Actin Santa Cruz Biotechnology Cat# sc-47778 Anti-mouse CD3 BioLegend Cat# 102102 Anti-mouse CD3 Novus Biologicals Cat# FAB4841G-100 Anti-mouse CD4 Novus Biologicals Cat# FAB554A-100 Anti-mouse CD8A Novus Biologicals Cat# NBP1-49045PE Anti-mouse CD11b Novus Biologicals Cat# NB110-89474AF405 Anti-mouse CD11c Novus Biologicals Cat# NB110-40766AF488 Anti-mouse CD25 Novus Biologicals Cat# NBP2-27425AF488 Anti-mouse CD28 BioLegend Cat# 100223 Anti-mouse CD44 Novus Biologicals Cat# NBP1-47386APC Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF488 Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF405 Anti-mouse CD69 Novus Biologicals Cat# NBP1-28011AF488 Anti-mouse CD80 Novus Biologicals Cat# NBP1-43385AF488 Anti-mouse CD314 Novus Biologicals Cat# FAB1547V-100UG Anti-mouse F4/80 Novus Biologicals Cat# NB600-404APC Anti-mouse FoxP3 Novus Biologicals Cat# NB100-39002PE Anti-human HIF-1a Novus Biologicals Cat# NB100-479 Anti-human HIF-1b Novus Biologicals Cat# NB100-124 Anti-human HIF-2a Novus Biologicals Cat# NB100-122 Anti-mouse IFNG Novus Biologicals Cat# IC485V-100UG Anti-mouse Ly6c Novus Biologicals Cat# NBP1-28046AF488 Anti-mouse Ly6g Novus Biologicals Cat# FAB1037A-100 Anti-mouse NK1.1 Novus Biologicals Cat# NB100-77528APC Anti-mouse p50 Novus Biologicals Cat# NBP2-6735 Anti-mouse Rel A Novus Biologicals Cat# NB100-2176 Anti-mouse Rel B Novus Biologicals Cat# NBP2-20123 Anti-mouse a-Tubulin Novus Biologicals Cat# NB600-506 Armenian Hamster IgG, anti-mouse CXCL9 (MIG) Bio X Cell Cat# BE0309 Polyclonal Armenian hamster IgG Bio X Cell Cat# BE0091 Syrian Hamster IgG, anti-mouse CTLA-4 Bio X Cell Cat# BP0131 Rat IgG2a, k, anti-mouse PD-1 (CD279) Bio X Cell Cat# BP0146 Rat IgG2a isotype control Bio X Cell Cat# BE0089 Chemicals, Peptides, and Recombinant Proteins Acriflavine Sigma Aldrich SKU # A8126 TRIzol Reagent Invitrogen Cat# 15596026 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 ECL Prime Western Blotting System GE Healthcare SKU# GERPN2232 PolyJet In Vitro DNA Transfection Reagent Signagen Cat # SL100688 Rabbit anti-mouse IgG-HRP Santa Cruz Biotech Cat# sc-358914 Rabbit IgG HRP Linked Whole Ab GE Healthcare SKU# GENA934 (Continued on next page) e1 Cell Reports 32, 108073, August 25, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase type I Sigma Aldrich Cat# SCR103 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) BD PharMingen Cat# 553141 Critical Commercial Assays High-Capacity RNA-to-cDNA Kit Applied Biosystem Cat# 4387406 Ambion DNase I (RNase-free) Invitrogen Cat# AM2222 iQ SYBR Green Supermix Bio-Rad Cat# 1708884 Mouse CXCL9/MIG Quantikine ELISA Kit R&D Cat# MCX900 RT2 ProfilerTM PCR Array of Mouse Cytokines & Chemokines QIAGEN Cat# PAMM-150ZD-6 Experimental Models: Cell lines MDA-MB-231 human breast cancer cell line ATCC ATCC HTB-26 4T1 mouse mammary carcinoma cell line ATCC ATCC CRL-2539 EMT-6 mouse mammary carcinoma cell line ATCC ATCC CRL-2755 B16F10 mouse melanoma cell line ATCC ATCC CRL-6475 293T human embryonic kidney cell line ATCC ATCC CRL-11268 Experimental Models: Organisms/Strains BALB/c female mice, 6-8 weeks old Charles River 028 C57BL/6 female mice, 6-8 weeks old Charles River 027 BALB/c Scid female mice, 6-8 weeks old The Jackson Laboratory 001803 Oligonucleotides m BIRC2 TGTGGCCTGATGTTGGATAAC ThermoFisher N/A m BIRC2 GGTGACGAATGTGCAAATCTACT ThermoFisher N/A m GPI TCAAGCTGCGCGAACTTTTTG ThermoFisher N/A m GPI GGTTCTTGGAGTAGTCCACCAG ThermoFisher N/A m ITGAV CCGTGGACTTCTTCGAGCC ThermoFisher N/A m ITGAV CTGTTGAATCAAACTCAATGGGC ThermoFisher N/A m MCL1 GACGACCTATACCGCCAGTC ThermoFisher N/A m MCL1 AGAGGCTTCGAGTCCTTGGA ThermoFisher N/A m NAMPT GCAGAAGCCGAGTTCAACATC ThermoFisher N/A m NAMPT TTTTCACGGCATTCAAAGTAGGA ThermoFisher N/A m SOD2 CAGACCTGCCTTACGACTATGG ThermoFisher N/A m SOD2 CTCGGTGGCGTTGAGATTGTT ThermoFisher N/A m UGP2 AGCAAAGCTATGTCTCAAGATGG ThermoFisher N/A m UGP2 GAGGCTGCTGTGGTAAGTATTT ThermoFisher N/A m RPL13A GGGCAGGTTCTGGTATTGGAT ThermoFisher N/A m RPL13A GGCTCGGAAATGGTAGGGG ThermoFisher N/A m TIGIT GAATGGAACCTGAGGAGTCTCT ThermoFisher N/A m TIGIT AGCAATGAAGCTCTCTAGGCT ThermoFisher N/A m LAG3 CTGGGACTGCTTTGGGAAG ThermoFisher N/A m LAG3 GGTTGATGTTGCCAGATAACCC ThermoFisher N/A m CD27 ATGCTTCGAGGATGGGGTG ThermoFisher N/A m CD276 CCAGGCTCTGGGGAAAAGG ThermoFisher N/A m 18S rRNA GTAACCCGTTGAACCCCATT ThermoFisher N/A m 18S rRNA CCATCCAATCGGTAGTAGCG ThermoFisher N/A h BIRC2 AGCACGATCTTGTCAGATTGG ThermoFisher N/A h BIRC2 GGCGGGGAAAGTTGAATATGTA ThermoFisher N/A h 18S rRNA CGGCGACGACCCATTCGAAC ThermoFisher N/A h 18S rRNA GAATCGAACCCTGATTCCCCGTC ThermoFisher N/A ChIP BIRC2 TCACACCTGTAATCCCAGCA ThermoFisher N/A (Continued on next page) Cell Reports 32, 108073, August 25, 2020 e2
Western Blot:Article Title: BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse BIRC2 Novus Biologicals Cat# NB100-56889 Anti-mouse b-Actin Santa Cruz Biotechnology Cat# sc-47778 Anti-mouse CD3 BioLegend Cat# 102102 Anti-mouse CD3 Novus Biologicals Cat# FAB4841G-100 Anti-mouse CD4 Novus Biologicals Cat# FAB554A-100 Anti-mouse CD8A Novus Biologicals Cat# NBP1-49045PE Anti-mouse CD11b Novus Biologicals Cat# NB110-89474AF405 Anti-mouse CD11c Novus Biologicals Cat# NB110-40766AF488 Anti-mouse CD25 Novus Biologicals Cat# NBP2-27425AF488 Anti-mouse CD28 BioLegend Cat# 100223 Anti-mouse CD44 Novus Biologicals Cat# NBP1-47386APC Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF488 Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF405 Anti-mouse CD69 Novus Biologicals Cat# NBP1-28011AF488 Anti-mouse CD80 Novus Biologicals Cat# NBP1-43385AF488 Anti-mouse CD314 Novus Biologicals Cat# FAB1547V-100UG Anti-mouse F4/80 Novus Biologicals Cat# NB600-404APC Anti-mouse FoxP3 Novus Biologicals Cat# NB100-39002PE Anti-human HIF-1a Novus Biologicals Cat# NB100-479 Anti-human HIF-1b Novus Biologicals Cat# NB100-124 Anti-human HIF-2a Novus Biologicals Cat# NB100-122 Anti-mouse IFNG Novus Biologicals Cat# IC485V-100UG Anti-mouse Ly6c Novus Biologicals Cat# NBP1-28046AF488 Anti-mouse Ly6g Novus Biologicals Cat# FAB1037A-100 Anti-mouse NK1.1 Novus Biologicals Cat# NB100-77528APC Anti-mouse p50 Novus Biologicals Cat# NBP2-6735 Anti-mouse Rel A Novus Biologicals Cat# NB100-2176 Anti-mouse Rel B Novus Biologicals Cat# NBP2-20123 Anti-mouse a-Tubulin Novus Biologicals Cat# NB600-506 Armenian Hamster IgG, anti-mouse CXCL9 (MIG) Bio X Cell Cat# BE0309 Polyclonal Armenian hamster IgG Bio X Cell Cat# BE0091 Syrian Hamster IgG, anti-mouse CTLA-4 Bio X Cell Cat# BP0131 Rat IgG2a, k, anti-mouse PD-1 (CD279) Bio X Cell Cat# BP0146 Rat IgG2a isotype control Bio X Cell Cat# BE0089 Chemicals, Peptides, and Recombinant Proteins Acriflavine Sigma Aldrich SKU # A8126 TRIzol Reagent Invitrogen Cat# 15596026 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 ECL Prime Western Blotting System GE Healthcare SKU# GERPN2232 PolyJet In Vitro DNA Transfection Reagent Signagen Cat # SL100688 Rabbit anti-mouse IgG-HRP Santa Cruz Biotech Cat# sc-358914 Rabbit IgG HRP Linked Whole Ab GE Healthcare SKU# GENA934 (Continued on next page) e1 Cell Reports 32, 108073, August 25, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase type I Sigma Aldrich Cat# SCR103 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) BD PharMingen Cat# 553141 Critical Commercial Assays High-Capacity RNA-to-cDNA Kit Applied Biosystem Cat# 4387406 Ambion DNase I (RNase-free) Invitrogen Cat# AM2222 iQ SYBR Green Supermix Bio-Rad Cat# 1708884 Mouse CXCL9/MIG Quantikine ELISA Kit R&D Cat# MCX900 RT2 ProfilerTM PCR Array of Mouse Cytokines & Chemokines QIAGEN Cat# PAMM-150ZD-6 Experimental Models: Cell lines MDA-MB-231 human breast cancer cell line ATCC ATCC HTB-26 4T1 mouse mammary carcinoma cell line ATCC ATCC CRL-2539 EMT-6 mouse mammary carcinoma cell line ATCC ATCC CRL-2755 B16F10 mouse melanoma cell line ATCC ATCC CRL-6475 293T human embryonic kidney cell line ATCC ATCC CRL-11268 Experimental Models: Organisms/Strains BALB/c female mice, 6-8 weeks old Charles River 028 C57BL/6 female mice, 6-8 weeks old Charles River 027 BALB/c Scid female mice, 6-8 weeks old The Jackson Laboratory 001803 Oligonucleotides m BIRC2 TGTGGCCTGATGTTGGATAAC ThermoFisher N/A m BIRC2 GGTGACGAATGTGCAAATCTACT ThermoFisher N/A m GPI TCAAGCTGCGCGAACTTTTTG ThermoFisher N/A m GPI GGTTCTTGGAGTAGTCCACCAG ThermoFisher N/A m ITGAV CCGTGGACTTCTTCGAGCC ThermoFisher N/A m ITGAV CTGTTGAATCAAACTCAATGGGC ThermoFisher N/A m MCL1 GACGACCTATACCGCCAGTC ThermoFisher N/A m MCL1 AGAGGCTTCGAGTCCTTGGA ThermoFisher N/A m NAMPT GCAGAAGCCGAGTTCAACATC ThermoFisher N/A m NAMPT TTTTCACGGCATTCAAAGTAGGA ThermoFisher N/A m SOD2 CAGACCTGCCTTACGACTATGG ThermoFisher N/A m SOD2 CTCGGTGGCGTTGAGATTGTT ThermoFisher N/A m UGP2 AGCAAAGCTATGTCTCAAGATGG ThermoFisher N/A m UGP2 GAGGCTGCTGTGGTAAGTATTT ThermoFisher N/A m RPL13A GGGCAGGTTCTGGTATTGGAT ThermoFisher N/A m RPL13A GGCTCGGAAATGGTAGGGG ThermoFisher N/A m TIGIT GAATGGAACCTGAGGAGTCTCT ThermoFisher N/A m TIGIT AGCAATGAAGCTCTCTAGGCT ThermoFisher N/A m LAG3 CTGGGACTGCTTTGGGAAG ThermoFisher N/A m LAG3 GGTTGATGTTGCCAGATAACCC ThermoFisher N/A m CD27 ATGCTTCGAGGATGGGGTG ThermoFisher N/A m CD276 CCAGGCTCTGGGGAAAAGG ThermoFisher N/A m 18S rRNA GTAACCCGTTGAACCCCATT ThermoFisher N/A m 18S rRNA CCATCCAATCGGTAGTAGCG ThermoFisher N/A h BIRC2 AGCACGATCTTGTCAGATTGG ThermoFisher N/A h BIRC2 GGCGGGGAAAGTTGAATATGTA ThermoFisher N/A h 18S rRNA CGGCGACGACCCATTCGAAC ThermoFisher N/A h 18S rRNA GAATCGAACCCTGATTCCCCGTC ThermoFisher N/A ChIP BIRC2 TCACACCTGTAATCCCAGCA ThermoFisher N/A (Continued on next page) Cell Reports 32, 108073, August 25, 2020 e2
In Vitro:Article Title: BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse BIRC2 Novus Biologicals Cat# NB100-56889 Anti-mouse b-Actin Santa Cruz Biotechnology Cat# sc-47778 Anti-mouse CD3 BioLegend Cat# 102102 Anti-mouse CD3 Novus Biologicals Cat# FAB4841G-100 Anti-mouse CD4 Novus Biologicals Cat# FAB554A-100 Anti-mouse CD8A Novus Biologicals Cat# NBP1-49045PE Anti-mouse CD11b Novus Biologicals Cat# NB110-89474AF405 Anti-mouse CD11c Novus Biologicals Cat# NB110-40766AF488 Anti-mouse CD25 Novus Biologicals Cat# NBP2-27425AF488 Anti-mouse CD28 BioLegend Cat# 100223 Anti-mouse CD44 Novus Biologicals Cat# NBP1-47386APC Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF488 Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF405 Anti-mouse CD69 Novus Biologicals Cat# NBP1-28011AF488 Anti-mouse CD80 Novus Biologicals Cat# NBP1-43385AF488 Anti-mouse CD314 Novus Biologicals Cat# FAB1547V-100UG Anti-mouse F4/80 Novus Biologicals Cat# NB600-404APC Anti-mouse FoxP3 Novus Biologicals Cat# NB100-39002PE Anti-human HIF-1a Novus Biologicals Cat# NB100-479 Anti-human HIF-1b Novus Biologicals Cat# NB100-124 Anti-human HIF-2a Novus Biologicals Cat# NB100-122 Anti-mouse IFNG Novus Biologicals Cat# IC485V-100UG Anti-mouse Ly6c Novus Biologicals Cat# NBP1-28046AF488 Anti-mouse Ly6g Novus Biologicals Cat# FAB1037A-100 Anti-mouse NK1.1 Novus Biologicals Cat# NB100-77528APC Anti-mouse p50 Novus Biologicals Cat# NBP2-6735 Anti-mouse Rel A Novus Biologicals Cat# NB100-2176 Anti-mouse Rel B Novus Biologicals Cat# NBP2-20123 Anti-mouse a-Tubulin Novus Biologicals Cat# NB600-506 Armenian Hamster IgG, anti-mouse CXCL9 (MIG) Bio X Cell Cat# BE0309 Polyclonal Armenian hamster IgG Bio X Cell Cat# BE0091 Syrian Hamster IgG, anti-mouse CTLA-4 Bio X Cell Cat# BP0131 Rat IgG2a, k, anti-mouse PD-1 (CD279) Bio X Cell Cat# BP0146 Rat IgG2a isotype control Bio X Cell Cat# BE0089 Chemicals, Peptides, and Recombinant Proteins Acriflavine Sigma Aldrich SKU # A8126 TRIzol Reagent Invitrogen Cat# 15596026 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 ECL Prime Western Blotting System GE Healthcare SKU# GERPN2232 PolyJet In Vitro DNA Transfection Reagent Signagen Cat # SL100688 Rabbit anti-mouse IgG-HRP Santa Cruz Biotech Cat# sc-358914 Rabbit IgG HRP Linked Whole Ab GE Healthcare SKU# GENA934 (Continued on next page) e1 Cell Reports 32, 108073, August 25, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase type I Sigma Aldrich Cat# SCR103 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) BD PharMingen Cat# 553141 Critical Commercial Assays High-Capacity RNA-to-cDNA Kit Applied Biosystem Cat# 4387406 Ambion DNase I (RNase-free) Invitrogen Cat# AM2222 iQ SYBR Green Supermix Bio-Rad Cat# 1708884 Mouse CXCL9/MIG Quantikine ELISA Kit R&D Cat# MCX900 RT2 ProfilerTM PCR Array of Mouse Cytokines & Chemokines QIAGEN Cat# PAMM-150ZD-6 Experimental Models: Cell lines MDA-MB-231 human breast cancer cell line ATCC ATCC HTB-26 4T1 mouse mammary carcinoma cell line ATCC ATCC CRL-2539 EMT-6 mouse mammary carcinoma cell line ATCC ATCC CRL-2755 B16F10 mouse melanoma cell line ATCC ATCC CRL-6475 293T human embryonic kidney cell line ATCC ATCC CRL-11268 Experimental Models: Organisms/Strains BALB/c female mice, 6-8 weeks old Charles River 028 C57BL/6 female mice, 6-8 weeks old Charles River 027 BALB/c Scid female mice, 6-8 weeks old The Jackson Laboratory 001803 Oligonucleotides m BIRC2 TGTGGCCTGATGTTGGATAAC ThermoFisher N/A m BIRC2 GGTGACGAATGTGCAAATCTACT ThermoFisher N/A m GPI TCAAGCTGCGCGAACTTTTTG ThermoFisher N/A m GPI GGTTCTTGGAGTAGTCCACCAG ThermoFisher N/A m ITGAV CCGTGGACTTCTTCGAGCC ThermoFisher N/A m ITGAV CTGTTGAATCAAACTCAATGGGC ThermoFisher N/A m MCL1 GACGACCTATACCGCCAGTC ThermoFisher N/A m MCL1 AGAGGCTTCGAGTCCTTGGA ThermoFisher N/A m NAMPT GCAGAAGCCGAGTTCAACATC ThermoFisher N/A m NAMPT TTTTCACGGCATTCAAAGTAGGA ThermoFisher N/A m SOD2 CAGACCTGCCTTACGACTATGG ThermoFisher N/A m SOD2 CTCGGTGGCGTTGAGATTGTT ThermoFisher N/A m UGP2 AGCAAAGCTATGTCTCAAGATGG ThermoFisher N/A m UGP2 GAGGCTGCTGTGGTAAGTATTT ThermoFisher N/A m RPL13A GGGCAGGTTCTGGTATTGGAT ThermoFisher N/A m RPL13A GGCTCGGAAATGGTAGGGG ThermoFisher N/A m TIGIT GAATGGAACCTGAGGAGTCTCT ThermoFisher N/A m TIGIT AGCAATGAAGCTCTCTAGGCT ThermoFisher N/A m LAG3 CTGGGACTGCTTTGGGAAG ThermoFisher N/A m LAG3 GGTTGATGTTGCCAGATAACCC ThermoFisher N/A m CD27 ATGCTTCGAGGATGGGGTG ThermoFisher N/A m CD276 CCAGGCTCTGGGGAAAAGG ThermoFisher N/A m 18S rRNA GTAACCCGTTGAACCCCATT ThermoFisher N/A m 18S rRNA CCATCCAATCGGTAGTAGCG ThermoFisher N/A h BIRC2 AGCACGATCTTGTCAGATTGG ThermoFisher N/A h BIRC2 GGCGGGGAAAGTTGAATATGTA ThermoFisher N/A h 18S rRNA CGGCGACGACCCATTCGAAC ThermoFisher N/A h 18S rRNA GAATCGAACCCTGATTCCCCGTC ThermoFisher N/A ChIP BIRC2 TCACACCTGTAATCCCAGCA ThermoFisher N/A (Continued on next page) Cell Reports 32, 108073, August 25, 2020 e2
Transfection:Article Title: BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse BIRC2 Novus Biologicals Cat# NB100-56889 Anti-mouse b-Actin Santa Cruz Biotechnology Cat# sc-47778 Anti-mouse CD3 BioLegend Cat# 102102 Anti-mouse CD3 Novus Biologicals Cat# FAB4841G-100 Anti-mouse CD4 Novus Biologicals Cat# FAB554A-100 Anti-mouse CD8A Novus Biologicals Cat# NBP1-49045PE Anti-mouse CD11b Novus Biologicals Cat# NB110-89474AF405 Anti-mouse CD11c Novus Biologicals Cat# NB110-40766AF488 Anti-mouse CD25 Novus Biologicals Cat# NBP2-27425AF488 Anti-mouse CD28 BioLegend Cat# 100223 Anti-mouse CD44 Novus Biologicals Cat# NBP1-47386APC Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF488 Anti-mouse CD45 Novus Biologicals Cat# NB100-77417AF405 Anti-mouse CD69 Novus Biologicals Cat# NBP1-28011AF488 Anti-mouse CD80 Novus Biologicals Cat# NBP1-43385AF488 Anti-mouse CD314 Novus Biologicals Cat# FAB1547V-100UG Anti-mouse F4/80 Novus Biologicals Cat# NB600-404APC Anti-mouse FoxP3 Novus Biologicals Cat# NB100-39002PE Anti-human HIF-1a Novus Biologicals Cat# NB100-479 Anti-human HIF-1b Novus Biologicals Cat# NB100-124 Anti-human HIF-2a Novus Biologicals Cat# NB100-122 Anti-mouse IFNG Novus Biologicals Cat# IC485V-100UG Anti-mouse Ly6c Novus Biologicals Cat# NBP1-28046AF488 Anti-mouse Ly6g Novus Biologicals Cat# FAB1037A-100 Anti-mouse NK1.1 Novus Biologicals Cat# NB100-77528APC Anti-mouse p50 Novus Biologicals Cat# NBP2-6735 Anti-mouse Rel A Novus Biologicals Cat# NB100-2176 Anti-mouse Rel B Novus Biologicals Cat# NBP2-20123 Anti-mouse a-Tubulin Novus Biologicals Cat# NB600-506 Armenian Hamster IgG, anti-mouse CXCL9 (MIG) Bio X Cell Cat# BE0309 Polyclonal Armenian hamster IgG Bio X Cell Cat# BE0091 Syrian Hamster IgG, anti-mouse CTLA-4 Bio X Cell Cat# BP0131 Rat IgG2a, k, anti-mouse PD-1 (CD279) Bio X Cell Cat# BP0146 Rat IgG2a isotype control Bio X Cell Cat# BE0089 Chemicals, Peptides, and Recombinant Proteins Acriflavine Sigma Aldrich SKU # A8126 TRIzol Reagent Invitrogen Cat# 15596026 Puromycin Dihydrochloride ThermoFisher Cat# A1113803 ECL Prime Western Blotting System GE Healthcare SKU# GERPN2232 PolyJet In Vitro DNA Transfection Reagent Signagen Cat # SL100688 Rabbit anti-mouse IgG-HRP Santa Cruz Biotech Cat# sc-358914 Rabbit IgG HRP Linked Whole Ab GE Healthcare SKU# GENA934 (Continued on next page) e1 Cell Reports 32, 108073, August 25, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER Collagenase type I Sigma Aldrich Cat# SCR103 Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block) BD PharMingen Cat# 553141 Critical Commercial Assays High-Capacity RNA-to-cDNA Kit Applied Biosystem Cat# 4387406 Ambion DNase I (RNase-free) Invitrogen Cat# AM2222 iQ SYBR Green Supermix Bio-Rad Cat# 1708884 Mouse CXCL9/MIG Quantikine ELISA Kit R&D Cat# MCX900 RT2 ProfilerTM PCR Array of Mouse Cytokines & Chemokines QIAGEN Cat# PAMM-150ZD-6 Experimental Models: Cell lines MDA-MB-231 human breast cancer cell line ATCC ATCC HTB-26 4T1 mouse mammary carcinoma cell line ATCC ATCC CRL-2539 EMT-6 mouse mammary carcinoma cell line ATCC ATCC CRL-2755 B16F10 mouse melanoma cell line ATCC ATCC CRL-6475 293T human embryonic kidney cell line ATCC ATCC CRL-11268 Experimental Models: Organisms/Strains BALB/c female mice, 6-8 weeks old Charles River 028 C57BL/6 female mice, 6-8 weeks old Charles River 027 BALB/c Scid female mice, 6-8 weeks old The Jackson Laboratory 001803 Oligonucleotides m BIRC2 TGTGGCCTGATGTTGGATAAC ThermoFisher N/A m BIRC2 GGTGACGAATGTGCAAATCTACT ThermoFisher N/A m GPI TCAAGCTGCGCGAACTTTTTG ThermoFisher N/A m GPI GGTTCTTGGAGTAGTCCACCAG ThermoFisher N/A m ITGAV CCGTGGACTTCTTCGAGCC ThermoFisher N/A m ITGAV CTGTTGAATCAAACTCAATGGGC ThermoFisher N/A m MCL1 GACGACCTATACCGCCAGTC ThermoFisher N/A m MCL1 AGAGGCTTCGAGTCCTTGGA ThermoFisher N/A m NAMPT GCAGAAGCCGAGTTCAACATC ThermoFisher N/A m NAMPT TTTTCACGGCATTCAAAGTAGGA ThermoFisher N/A m SOD2 CAGACCTGCCTTACGACTATGG ThermoFisher N/A m SOD2 CTCGGTGGCGTTGAGATTGTT ThermoFisher N/A m UGP2 AGCAAAGCTATGTCTCAAGATGG ThermoFisher N/A m UGP2 GAGGCTGCTGTGGTAAGTATTT ThermoFisher N/A m RPL13A GGGCAGGTTCTGGTATTGGAT ThermoFisher N/A m RPL13A GGCTCGGAAATGGTAGGGG ThermoFisher N/A m TIGIT GAATGGAACCTGAGGAGTCTCT ThermoFisher N/A m TIGIT AGCAATGAAGCTCTCTAGGCT ThermoFisher N/A m LAG3 CTGGGACTGCTTTGGGAAG ThermoFisher N/A m LAG3 GGTTGATGTTGCCAGATAACCC ThermoFisher N/A m CD27 ATGCTTCGAGGATGGGGTG ThermoFisher N/A m CD276 CCAGGCTCTGGGGAAAAGG ThermoFisher N/A m 18S rRNA GTAACCCGTTGAACCCCATT ThermoFisher N/A m 18S rRNA CCATCCAATCGGTAGTAGCG ThermoFisher N/A h BIRC2 AGCACGATCTTGTCAGATTGG ThermoFisher N/A h BIRC2 GGCGGGGAAAGTTGAATATGTA ThermoFisher N/A h 18S rRNA CGGCGACGACCCATTCGAAC ThermoFisher N/A h 18S rRNA GAATCGAACCCTGATTCCCCGTC ThermoFisher N/A ChIP BIRC2 TCACACCTGTAATCCCAGCA ThermoFisher N/A (Continued on next page) Cell Reports 32, 108073, August 25, 2020 e2
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